FIGURE 5.
Carboxy-terminal peptides of Y14 in trans promote LLPS of truncated Y14. (A) Schematic diagram shows the sequence of N2 and C regions of human Y14 (upper), and the peptides derived from the carboxy-terminal domain of three Y14 homologs and the amino-terminal IDR of HPV E7 protein (E7N20). (B) The LLPS assay was performed with indicated peptide alone (20 µM) or in a mixture containing ΔN2 or ΔC (20 µM) in a 50 mM KCl pH 6.5 buffer. Representative DIC images are shown. Bar, 5 µm. N = 2. (C) The droplet size of truncated Y14/peptide mixtures was measured. Mean and standard deviation were calculated for 50 droplets each.
