FIGURE 5.
Interactions of pseudouridine H1N1 imino groups with bound water. Comparison of the 1D imino proton spectra (positive signals) and the corresponding CLEANEX-PM spectra (negative signals) for the unmodified WT NSR (top), the U14ψ variant (second row from top), the U13ψ variant (second row from bottom), and the U18ψ variant (bottom) of the WT NSR. Signal positions of the respective pseudouridine H1 imino protons are indicated in red lettering, other relevant signal assignments are given in black. Significant NOEs between H1 imino protons and water are highlighted with light red boxes.
