Transcriptome-wide analyses of piRNA binding sites suggest distinct mechanisms regulate piRNA binding and silencing in C. elegans

Secondary WAGO 22G-RNAs are preferentially produced in coding regions. (A) The density of WAGO-9 (top) and WAGO-1 (bottom) 22G-RNAs in the indicated regions of WAGO targets (left) or CSR-1 (right) targets. Blue lines represent median values. The statistical significance (P-value) of the difference in density between two regions was calculated by the Mann–Whitney U-test. (B) The ratio of WAGO-1 22G-RNAs in the prg-1 mutant over those in wild type in the indicated regions of WAGO (left) or CSR-1 (right) targets. Blue lines represent median values. The statistical significance (P-value) of the difference in fold change (prg-1 mutant/WT) between two regions was calculated by the Mann–Whitney U-test.