In vivo piRNA binding sites are enriched in the coding region (CDS) of germline mRNAs in C. elegans. (A) Metagene trace shows the distribution of predicted piRNA targeting sites along a germline mRNA. The solid line indicates the average number of piRNA targeting sites in all germline mRNAs. The dotted lines indicate the average number plus/minus one standard error. (B) The density of predicted piRNA targeting sites in the indicated regions of germline mRNAs. Blue lines represent median values. The statistical significance (P-value) of the difference in density between two regions was calculated by the Mann–Whitney U-test. (C) Metagene trace shows the distribution of experimentally identified piRNA binding sites along a germline mRNA. The solid line indicates the average number of piRNA binding sites in all germline mRNAs. The dotted lines indicate the average number plus/minus one standard error. (D) The density of experimentally identified piRNA binding sites in the indicated regions of germline mRNAs. Blue lines represent median values. The statistical significance (P-value) of the difference in density between two regions was calculated by the Mann–Whitney U-test. (E) The distribution of piRNA binding sites around the start codon (left) or stop codon (right). A 200 nt window centered at start or stop codon is shown. The solid line indicates the average number of piRNA binding sites in all germline mRNAs. For each position, the average is calculated from all germline mRNAs that possess that position.
