
Deconvoluted MS/MS sequencing spectrum U(m5C)(m5C)C(Um)G of Ini-tRNA-CAU from S. acidocaldarius after T1 and BAP digestion (m/z = 925.13, z = −2). The spectrum clearly shows the ion fragments corresponding to U47 without phosphate (c1 = 305 and y5 = 1545). See Table 2 for localization of the fragment.










