Identification of Up47 in three thermophilic archaea, one mesophilic archaeon, and one hyperthermophilic bacterium

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FIGURE 1.
FIGURE 1.

Deconvoluted MS/MS sequencing spectrum (Wolff et al. 2020) (UP)(m5C)(m5C)C(Um)Gp of Ini-tRNA-CAU from S. acidocaldarius after T1 digestion (m/z = 1005.2, z = −2). No ion fragment of Up is detected, probably due to the protection conferred by 2′-phosphate and insufficient CID energy, but ions c2 (704) and y4 (1306) corresponding to the dinucleotide (Wolff et al. 2020) were detected. See Table 2 for localization of the fragment.

This Article

  1. Published in Advance February 9, 2023, doi: 10.1261/rna.079546.122 RNA 29: 551-556

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