FIGURE 4.
(A) Proposed secondary structure of the Cm U5 snRNA. Cm U5 (dark red) is overlaid on yeast U5 snRNA (black). Model was generated from the MFold RNA secondary structure prediction web server (Zuker 2003). (B) Proposed model for interaction between snRNAs and all sequence variants of 5′ splice sites in Cm. The 5′ splice site of the pre-mRNA is indicated (middle); complementary region of U5 snRNA is shown above, and that of U6 snRNA is shown below. Watson–Crick base pairs, G–U pairs, and G–A base pairs are indicated with symbols accordingly. Interactions with U5 and U6 snRNAs are proposed to occur sequentially.
