DRBD18 associates with a subset of transcripts whose translational it regulates. Ten mRNAs translationally repressed by DRBD18 knockdown (black numbers) and six mRNAs translationally enhanced by DRBD18 knockdown (red numbers) were quantified in DRBD18 immunoprecipitations by qRT-PCR. Note that the primers targeting Tb927.6.760 also target Tb927.6.790, and those targeting Tb09.v4.0009 also target Tb927.9.15660. Values shown are fold enrichment in the DRBD18 pull-down compared to a blank bead control. Red line indicates no enrichment. The experiment was performed in biological triplicate, each with three technical qRT-PCR triplicates, and significance was calculated by Student's t-test. ns, not significant; (*) P < 0.05; (**) P < 0.01, (***) P < 0.001. Numbers indicate gene numbers from TriTrypDB.
