DRBD18 contributes to the maintenance of the procyclic translatome by repressing the translation of metacyclic-specific and enhancing the translation of procyclic-specific transcripts. (A) An available data set of protein abundances in procyclic versus metacyclic life cycle stages (Christiano et al. 2017) was analyzed for correlation with our TE data set. Transcripts were segregated by TE DOWN or TE UP, and the protein fold change (FC) in metacyclics versus procyclics was plotted for each gene that was present in the proteome. (**) P = 0.0063 by Student's t-test. Red dots, nine TE DOWN transcripts with metacyclic (MF)/procyclic (PF) FC values of <0.5, and seven TE UP transcripts with MF/PF FC values of >2.0. (B) Venn diagram showing the intersection of TE UP and metacyclic (MF) up genes; identities of the seven overlapping genes are shown to the right. (C) Venn diagram showing the intersection of TE DOWN and MF down genes; identities of the nine overlapping genes are shown to the right. In (B) and (C), hypergeometric tests were performed to assess significance of these intersections.
