DRBD18 contributes to the maintenance of the procyclic translatome by repressing the translation of bloodstream-specific and enhancing the translation of procyclic-specific transcripts. (A) An available data set of protein abundances (Tinti and Ferguson 2022) in procyclic versus bloodstream life cycle stages is plotted with genes from the TE UP and TE DOWN categories highlighted in magenta and yellow, respectively. A cutoff value of 3.7 on each axis was arbitrarily selected to enrich protein lists in procyclic- and bloodstream-specific proteins. (B) Venn diagram of the intersection between the TE DOWN category and procyclic (PF) specific proteins. A hypergeometric test was performed to assess the significance of these intersections. A list of intersecting genes is shown below. (C) Venn diagram of the intersection between the TE UP genes and bloodstream (BF) specific proteins. A hypergeometric test was performed to assess the significance of these intersections. A list of intersecting genes is shown below. (D) Western blot of RPB10 (Tb927.8.2780), confirming its up-regulation upon DRBD18 knockdown in PFs. P22 is a loading control.
