METTL16 destabilizes MAT2A mRNA. (A) Western blot showing knockdown of METTL16 upon treatment with METTL16 siRNAs (siM16) compared to nontargeting control siRNA (siNT). PABPN1 serves as a loading control. (B) Representative northern blots with RNA from cells transfected with the indicated siRNA and the indicated reporters. GFP signal serves as a control for transfection and loading. The asterisk denotes hyperadenylated transcripts as described in Figure 4C. (C) Quantification of the decay experiments as represented in panel B (n = 4). The data were analyzed by a two-tailed, unpaired Student's t-test comparing siNT to siM16 for each time point ([*] P < 0.05, [**] P < 0.01).
