FIGURE 3.
Strengthening the PPT bypasses the activity of hp1, but not hp2–6. (A) Representative northern blot of expression of β-MAT reporters with wild-type or mutant PPT constructs. The two A → U mutations are underlined; one G remains in the PPT, perhaps explaining the low levels of intron detention that persists in the mhp1 constructs. (B) Northern blot data were quantified as %DI. (C) Northern blot data were quantified to show relative mRNA levels. Each value was first normalized to signal from a cotransfected GFP loading control and expressed relative to the wild-type PPT sample after 2 h in −Met media. Bar graphs in B and C show mean values ±SD (n = 3).
