Direct dsRNA preparation by promoter-free RCT and RNase H cleavage using one circular dsDNA template with a mismatched bubble

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FIGURE 3.
FIGURE 3.

Effects of NTP concentration, dsDNA template concentration, and the addition of IPP on pfRCT. (A) NTP concentration. Other conditions: [T-B15] = 50 nM, without IPP, 24 h, 37°C. Analyzed by 1% agarose. (B) Time courses under various concentrations of each NTP. Other conditions: [T-B15] = 50 nM, with 1 U/mL IPP. (C) Time courses under various template concentrations, with or without IPP. [NTP]each = 2 mM, [IPP] = 0 or 1 U/mL. The reaction conditions are shown in Materials and Methods.

This Article

  1. Published in Advance August 3, 2023, doi: 10.1261/rna.079670.123 RNA 29: 1691-1702

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