Closely packed ribosomes sensitive to 1,6-hexanediol treatment are detectable in the cytoplasm of A549 cells. (A) Typical example of a ring-like ribosome structure revealed by TEM after fixing with glutaraldehyde (top) and diagram of quantification of combined results of three independent experiments where ring ribosomes were counted on at least 1500 µm3 cell space for each condition analyzing at least three cells/biological replicates (with/without HEX) (below). Statistical significance is determined by two-tailed Student's t-test ([*] P < 0.05). Scale bar, 50 nm. (B) Cells were fixed with glutaraldehyde and examined with TEM. Scale bar, 500 nm. White arrows indicate typical ring-like ribosome structures, the most common appearance of neighboring ribosomes in nontreated cells. (C) Cells were treated with HEX for 1 h and fixed with glutaraldehyde for TEM as in panel B. Same magnification as in panel B. White arrows indicate ribosomes arranged in a linear fashion, the most common appearance of neighboring ribosomes in HEX-treated cells.
