Permutation test reveals that mRNAs in EDTA-pellets are enriched in amino-terminally disordered protein-encoding ones. Taking 10,000 times randomly 3000 proteins from the UniProt database leads to a normal distribution of counts where each value at the x-axis represents the ratio of amino-terminally disordered and nondisordered protein counts. Protein disorder propensity at their 25 amino acid long amino-terminal was determined using the AlphaFold database (Jumper et al. 2021). Vertical line represents the ratio of disordered and nondisordered protein counts encoded by the 3000 mRNAs that were selected previously as mRNAs sedimenting in EDTA-pellet based on their pellet/EDTA-pellet read count ratio.
