SAFB associates with nascent RNAs and can promote gene expression in mouse embryonic stem cells

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FIGURE 5.
FIGURE 5.

Reintroduction of SAFB into SAFB/2 knockout cells restores gene expression defects in a manner dependent on the SAFB carboxy-terminal domain. (A) Protein domain diagram of SAFB (top) and ΔDD3 (bottom). IUPred2 disorder predictions below. (B) αFLAG western blot of GFP and SAFB rescue in DKO ESCs. (C) αV5 and αFLAG RIP-qPCR signal relative to FL-SAFB in FL-SAFB, ΔDD3, and GFP rescue cells. (D,E) Boxplots of average TPM in FL-SAFB, ΔDD3, and GFP rescue cells. (*) P < 0.05, (**) P < 0.01, (***) P < 0.001, respectively.

This Article

  1. Published in Advance July 19, 2023, doi: 10.1261/rna.079569.122 RNA 29: 1535-1556

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