SAFB/2 loss induces changes in splicing that are largely independent of the changes it induces in gene expression. (A) Venn diagram showing the numbers of genes that exhibit differential expression, alternative splicing events (ASE), or both, upon knockout of SAFB/2. (B) The number of significant events (Padj < 0.05) and histograms displaying the relative PSI values for each of the five different classes of ASE detected by rMATS (Shen et al. 2014). “Inc. Level Diff,” Inclusion Level Difference between WT and DKO ESCs. (C) Same as (B) but displaying relative PSI values and significant events (Padj < 0.05) when GFP-expressing DKO cells are compared to SAFB-expressing DKO cells. (*) P < 0.05; (**) P < 0.01.
