SAFB associates with nascent RNAs and can promote gene expression in mouse embryonic stem cells

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FIGURE 1.
FIGURE 1.

Generation and validation of SAFB/2 double-knockout mouse ESCs. (A) Schematic of Safb and Safb2 mm10 genomic locus with location of sgRNAs (g8–g12) and genotyping primers (13F/11R; 41R/44F; 59F/60R). (B) Sanger sequencing traces through the deleted region in the two clonal knockout lines used in this study, DKO4 and DKO13. (C) PCR genotyping in wild-type (WT) and DKO ESCs. (D) qPCR for RNA detection of Safb and Safb2 mRNA levels (shown relative to GAPDH in WT cells). Dots represent technical triplicate measurements. (E) Western blots showing levels of SAFB and SAFB2 in WT and DKO cells.

This Article

  1. Published in Advance July 19, 2023, doi: 10.1261/rna.079569.122 RNA 29: 1535-1556

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