Spatial arrangement of functional domains in OxyS stress response sRNA

NMR structural characterization of SL3. (A) ¹H–¹⁵N SF-HMQC spectrum acquired at 5°C. (B) Imino–imino region of JR-NOESY spectrum (τ_m = 200 msec) acquired at 5°C. Imino–imino contacts are denoted with blue lines. (C) Aromatic–anomeric region of NOESY spectrum (τ_m = 250 msec) with traced sequential connectivities, acquired at 25°C. (D) Secondary structure of SL3. Overhangs at 5′- and 3′-end are in black. Spectra were acquired at 1 mM RNA concentration, 15 mM Na-phosphate buffer, 30 mM NaCl, and pH 6 in 90% H₂O and 10% ²H₂O on a 600 MHz NMR spectrometer.