Northern blot analysis of 6S-1 RNA processing and decay in the parental B. subtilis W168 strain (wt) using probes against different portions of 6S-1 RNA. (A) Hybridization with probes 6S-1_mature, 6S-1_5′-half and 6S-1_3′-half. (B) Hybridization with probe 6S-1_a. (C) Hybridization with probes 6S-1_b to d; in the blot on the right, 12% (instead of 10%) denaturing PAGE was used to resolve fragments as short as 25 nt. The shown blots in panels A–C are representative of two individual blots performed with independent RNA preparations. For details on the probes, see Figure 1 and Table 1. For T7 transcripts used as size markers and to control probe specificity, see Supplemental Methods; M 3′-6S-1 (25 nt) and M 3′-6S-1 (50 nt) are DNA oligonucleotides identical in sequence to the last 25 or 50 nt of 6S-1 RNA (see Fig. 1).
