Processing and decay of 6S-1 and 6S-2 RNAs in Bacillus subtilis

(A) Northern blot analysis using probe 6S-1_5′-half to determine the identity of the shorter 6S-1 RNA fragments. Lanes 1–3: as lanes 1,3,4 in Figure 2A. In addition to the 208- and 197-nt transcripts (lanes 6,7), we further loaded T7 transcripts mimicking the 5′- and 3′-halves of 6S-1 RNA (lanes 4,5; for details, see Supplemental Methods). (B) As in panel A, but using probe pre-6S-1 to specifically detect 6S-1 RNA species containing the 5′-precursor segment (11 nt; see Fig. 1). The shown blots are representative of four (panel A) and seven (panel B) individual blots using two to four independent RNA preparations.