Antisense probes used for the detection of B. subtilis 6S-1 RNA and fragments thereof in northern blot experiments. The probes are illustrated as colored lines along the secondary structure of 6S-1 RNA; sky blue line: LNA/DNA mixmer probe to specifically detect 6S-1 RNA precursor molecules (probe pre-6S-1); dark blue line: antisense 6S-1 RNA (probe 6S-1_mature); orange lines: RNAs antisense to roughly the 5′-half (5′, probe 6S-1_5′-half) or 3′-half (3′, probe 6S-1_3′-half) of 6S-1 RNA; green lines: LNA/DNA mixmer probes (#a–d, probes 6S-1_a–d) for the detection of 6S-1 RNA fragments derived from the first to the fourth quarter of the RNA. 5′-CB, 3′-CB: 5′- and 3′-parts of the CB. For further details, see Materials and Methods.
