Ro causes dose-dependent stress granule formation. (A) Correlation between gene expression changes in Ro-treated H295R cells and gene expression changes from RBP knockdown in K562 cells (ENCODE) for the subset of genes with Ro-dependent expression changes (see Materials and Methods). RBPs colored by the significance of the correlation coefficient. (Pink) Positive, (gray) not significan, (blue) negative. (B) CDF plot of the log2 fold change in expression between Ro- and DMSO-treated cells for stress granule-localized transcripts (red) and all other transcripts (black). The Kolmogorov–Smirnov test was used to calculate statistical significance. (C) Overlap of Ro up-regulated transcripts and stress granule enriched transcripts. A hypergeometric test was used to calculate statistical significance. (D) Immunofluorescence of a stress granule marker, HuR/ELAVL1 (green), for DMSO- or Ro-treated H295R cells. Nuclei are shown in blue.
