Trim71-mediated repression of Ago2. (A) Western blotting in the WT and Trim71-dTAG mESCs at the indicated time points after dTAG-13 (500 nM) treatment. Gapdh protein levels were used for normalization in protein quantification. (B) Quantification of Ago2 protein levels in the WT and Trim71-dTAG mESCs at the indicated time points after dTAG-13 treatment. (C) Quantification of Ago2 mRNA levels in the WT and Trim71-dTAG mESCs at the indicated time points after dTAG-13 treatment. Gapdh mRNA was used for normalization in the quantification. The results represent the means (±SD) of three independent experiments. (D) Polysome analysis of the Trim71-dTAG mESCs with the dTAG-13 (500 nM) treatment at 0, 8, and 24 h. The target mRNA distribution in the polysome fractions was quantified by qRT-PCR. The quantifications from B to D represent the means (±SD) of three independent experiments. (*) P < 0.05 by one-way ANOVA.
