Identification of RNA-binding proteins’ direct effects on gene expression via the degradation tag system

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

FIGURE 1.
FIGURE 1.

An inducible protein degradation system for Trim71. (A) Schematic depiction of the dTAG system for degrading Trim71. (B) Workflow for knock-in the dTAG degrader to the Trim71 locus. (C) Genotyping of the Trim71-dTAG mESCs using the two primers in (B). (D) Western blotting in the WT and Trim71-dTAG mESCs. Gapdh protein levels were used for normalization in protein quantification. (*) Nonspecific bands.

This Article

  1. Published in Advance July 6, 2023, doi: 10.1261/rna.079669.123 RNA 29: 1453-1457

Current Issue

  1. July 2026, 32 (7)
  1. Current Issue
  1. Alert me to new issues of RNA