Detection of up- or down-regulated genes. (A) TSC22D3 (GILZ) transcript abundance as HCR spots/cell (blue) after in vitro treatment of human primary B cells with vehicle (0.1% ethanol) or methylprednisolone (MP) (200 µg/dL) for 2 h. Magnification: 60×. Scale bar for full FOV: 20 microns. Scale bar for inset: 10 microns. (B) Density plots showing the distributions of TSC22D3 transcript abundance after MP or vehicle treatment. (C) TNF transcript abundance as HCR spots/cell (green), after in vitro stimulation of human primary monocytes with LPS (1 ng/mL) for 30 min, followed by treatment with vehicle (0.1% ethanol) or MP (200 µg/dL) for 2 h. Magnification: 60×. Scale bar for full FOV: 20 microns. Scale bar for inset: 10 microns. (D) Density plots showing the distributions of TNF transcript abundance after LPS stimulation followed by vehicle or MP treatment.
