FIGURE 2.
The sequences and secondary structures of each RNA segment studied in this work. Three smaller constructs, corresponding to independently folded secondary structure domains, were prepared to facilitate structure determination: DenvBS represents the bottom helix; DenvTSL the top stem–loop; and DenvSLAsh the three-way junction. Secondary structures for each segment were predicted using the UNAFold web server (http://www.unafold.org) (Markham and Zuker 2008; Zuker 2003) and verified by the NMR assignments.
