FIGURE 1.
The sequence and secondary structure of the SLA promoter element that recruits the NS5 protein to initiate minus-strand RNA synthesis is very highly conserved among the four dengue virus serotypes (DENV1-4). (A) Alignment of the sequences of the first 70 nt of DENV1, DENV2, DENV3, and DENV4. The regions corresponding to the predicted secondary structure elements (bottom helix, top and side stem–loops) are indicated at the top. Conserved nucleotides are shaded. (B) Predicted RNA secondary structure of the SLA for the four DENV serotypes, as confirmed by SHAPE (Dethoff et al. 2018).
