Targeting the noncoding RNA component of RNase P. (A) Structure of the T. maritima RNase P RNA (rnpB RNA in cyan) in complex with the RnpA protein (purple) and substrate tRNA (orange) (PDB 3Q1R). (B) The relative positions of each tested crRNA (1–7) are indicated on the lower diagram and are superimposed (red) onto the rnpB RNA structure (cyan). (C,E) Expression of the L. lactis type III-A crRNP containing a crRNA targeting rnpB (1–7) was induced, and northern analysis was performed with probes against the rnpB RNA (C), valV-valW pre-tRNA (E), or 5S rRNA (C,E). The positions of the rnpB RNA full-length (dot) and cleavage products (red stars) and sizes of molecular weight markers (M) are indicated. (D) Schematic of the valV-valW pre-tRNA processing by RNase P and binding location of probe used to selectively recognize the unprocessed pre-tRNA transcript (E).
