FIGURE 3.
Type III-A crRNPs can cleave at distinct sites along the length of a mRNA. (A) Diagram of the lpp mRNA showing the relative positions of each tested crRNA and northern probes used. (B,C) Expression of the L. lactis type III-A crRNPs containing different crRNAs (1–5) was induced, and northern analysis was performed using probes against the 5′ (B) and 3′ (C) termini of the lpp mRNA. The positions of the full-length lpp mRNA (dot) and cleavage products (red star) are indicated in each panel. The sizes of the molecular weight markers (M) are indicated in each panel.
