Targeted mRNA destruction by S. thermophilus and S. epidermidis type III-A crRNPs. (A–C) Expression of S. thermophilus type III-A crRNPs containing either a crRNA against the lpp mRNA (lpp) or negative control crRNA (C) was induced (+), and northern analysis was performed using probes against the lpp mRNA (A), lpp crRNA (B), control crRNA (C), or 5S rRNA (B,C). (D) Expression of the S. epidermidis (Sep) and L. lactis (Lla) type III-A crRNPs containing either a crRNA against the lpp mRNA or control crRNA was induced (+), and northern analysis was performed using probes against the lpp mRNA and 5S rRNA. The positions of the RNAs are indicated, including those of the full-length lpp mRNA (dot) and lpp mRNA cleavage products (red star). (A–C) The dotted lines indicate intervening lanes were omitted from the blot. The sizes of the molecular weight markers (M) are indicated in each panel.
