FIGURE 4.
Effects on translational frameshifting in vivo of various KO strains. (A) Drawing of the dual luciferase fusion gene (top; positive control) and the sequences of five different frameshifting mutants (bottom; see Materials and Methods). (B–G) Ratio of Firefly and Renilla luciferase luminescence signals for the indicated strains carrying a plasmid from A. B used the top sequence in A while G used the 11-nt mutated region. Standard errors of at least five biological replicates are given. P < 0.05 for ΔCKLNMuE vs. WT in C, D, F, and G; for ΔrlmE vs. WT in B, C, D, F, and G; for ΔrluC/ΔrlmE vs. WT in C, D, E, and G; for ΔrluC/ΔrlmE v ΔrlmE in B.
