Splicing efficiency of minor introns in a mouse model of SMA predominantly depends on their branchpoint sequence and can involve the contribution of major spliceosome components

Time course analyses of splicing reactions performed with the Tsta3 or Tsta3 BPS Slc12a4 reporter pre-mRNAs. (A) Time course analysis of the Tsta3 or Tsta3 BPS Slc12a4 pre-mRNAs splicing was performed at 0, 5, 10, 15, and 20 min time points (left panels) and at 0, 20, 40, 60, 80, and 100 min time points (right panels). Splicing products were separated in 4% denaturing polyacrylamide gels. The schematic structure of the splicing products is indicated. (B,C) Splicing efficiency (in %) of the Tsta3 and Tsta3 BPS Slc12a4 pre-mRNAs (n = 2).