Differential splicing of minor introns in spinal cords of SMA mice. (A) RT-PCR analysis of minor introns splicing in spinal cords of control (WT6, WT7) and SMA (KO6, KO9, KO10) mice. Three examples of retained introns are shown. Gene names and size (in base pairs) of the amplified products are indicated on the left. The schematic structure of the amplified products is shown on the right. (B) RT-PCR analysis of minor introns splicing in spinal cords of control (WT6, WT7) and SMA (KO6, KO9, KO10) mice. Three examples of unaffected introns are shown. (C) Retention index of minor introns in spinal cords of control (WT, n = 2) and SMA (SMA, n = 3) mice determined as described in the Materials and Methods section. Mean and SEM are shown. Statistical significance was calculated using the Student t-test (ns: nonsignificant; (*) P < 0.05). Histograms for Ppp2r2b and 2c are not shown because retained introns are not detectable.
