Three critical regions of the erythromycin resistance methyltransferase, ErmE, are required for function supporting a model for the interaction of Erm family enzymes with substrate rRNA

Sequence conservation analysis of Εrm from clinical samples. Sequence logos were generated from pathogen-derived Erm sequences to analyze sequence variation across the adenosine pocket, α4 cleft, and basic ridge. Sequences were obtained from the NCBI MicroBIGG-E, a database of antimicrobial resistance genes derived from next-generation sequencing data of clinical samples and samples associated with foodborne disease outbreaks. Subtypes are indicated at left; MBE refers to all MicroBIGG-E sequences.