Three critical regions of the erythromycin resistance methyltransferase, ErmE, are required for function supporting a model for the interaction of Erm family enzymes with substrate rRNA

Superpositions of human rRNA adenine dimethylase family members and ErmC onto ErmE highlighting structural variation in the α4 cleft. (A) A superposition of human TFB1M given by pdb code 6aax, onto our ErmE–rRNA model. The view emphasizes the α4 cleft region. (B) A superposition of human DIMT1, given by pdb code 6w6c, onto our ErmE–rRNA model. (C) A superposition of bacterial ErmC, given by pdb code 1qao, onto our ErmE–rRNA model. In all cases only the catalytic domains of the enzymes are shown for simplicity.