E. coli 6S RNA complexed to RNA polymerase maintains product RNA synthesis at low cellular ATP levels by initiation with noncanonical initiator nucleotides

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FIGURE 7.
FIGURE 7.

NAD+ capping efficiency is strongly modulated by 6S RNA sequence in vitro. (A) 6S RNA with A45 underlined. (B) Competitive in vitro pRNA synthesis using A45N 6S RNA templates with NAD+ concentration held constant at 1 mM and ATP decreasing from 100 to 10 to 1 µM. Samples were normalized by pRNA specific activity and resolved using 23% denaturing PAGE containing 0.25% APB.

This Article

  1. Published in Advance October 5, 2022, doi: 10.1261/rna.079356.122 RNA 28: 1643-1658

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