E. coli 6S RNA complexed to RNA polymerase maintains product RNA synthesis at low cellular ATP levels by initiation with noncanonical initiator nucleotides

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

FIGURE 7.
FIGURE 7.

NAD+ capping efficiency is strongly modulated by 6S RNA sequence in vitro. (A) 6S RNA with A45 underlined. (B) Competitive in vitro pRNA synthesis using A45N 6S RNA templates with NAD+ concentration held constant at 1 mM and ATP decreasing from 100 to 10 to 1 µM. Samples were normalized by pRNA specific activity and resolved using 23% denaturing PAGE containing 0.25% APB.

This Article

  1. Published in Advance October 5, 2022, doi: 10.1261/rna.079356.122 RNA 28: 1643-1658

Current Issue

  1. July 2026, 32 (7)
  1. Current Issue
  1. Alert me to new issues of RNA