Activity of monopartite methylation guide RNAs. (A) Secondary structure diagrams of MMG1 to MMG5. These RNAs share the same guide sequence as the D guide of sR1c and BMG2. The pairing of MMG1 with substrates S8–S10 are shown. Modification targets are indicated by circles. (B–H) Activity and specificity of Ss RNPs assembled with sR1c (B), BMG2 (C), MMG1 (D), MMG2 (E), MMG3 (F), MMG4 (G), and MMG5 (H) and without (w/o) or with (w/) L7Ae. Substrates S8–S10 or their premethylated versions (sub-m) were modified at 70°C for 20 min. Reactions from a same gel are boxed. Relative modification levels were quantified against the reference reaction (Ref) present in each gel that showed modification of S10 by sR1c holo-RNP.
