Coligo-seq data summary for Pol III transcription of coligos mut2B, 122, 221, and 143-2. (A) Denaturing polyacrylamide gels showing in vitro transcript profiles. The RNA within the dashed line rectangles was cut from the gel for coligo-seq. Spiral arrows: tandem multimer transcripts. IVT, in vitro transcription. M, RNA marker. A line between lanes indicates lanes originally separated on the same gel. (B) Pol III initiation sites (green bars) and termination sites (red bars) on coligo mut2B from compiled coligo-seq reads mapped onto the sequence illustrated with its predicted secondary structure. (C) Coligo-seq results for coligo 122, as in panel B. A total of 35.99% of transcripts began at the -TTT- sequence with the following 5′ RNA end distributions: 5′rAA (7.27%); 5′rAAA (4.83%); 5′rAAAA (18.91%); and 5′rAAAAA (4.98%). Untemplated rA nt in the latter two transcripts may indicate Pol III slippage during initiation. (D) Coligo-seq results for coligo 221, as in panel B. (E) Coligo-seq results for coligo 143-2, as in panel B.
