FIGURE 1.
The schematic of the nanoluciferase pretermination complex. (A) Crystal structure of nanoluciferase. Carboxy-terminal amino acids (129–169; red) likely occupy the polypeptide tunnel of the ribosome in the pretermination complex. (B) mRNA constructs used to monitor nanoluciferase release. (C) Progress of nanoluciferase mRNA translation in rabbit reticulocyte lysate: stop codon containing mRNA is shown in green and nonstop mRNA translation followed by addition of puromycin is shown in yellow (relative luminescence units [RLUs] are on the y-axis).
