Biochemical and structural bioinformatics studies of fungal CutA nucleotidyltransferases explain their unusual specificity toward CTP and increased tendency for cytidine incorporation at the 3′-terminal positions of synthesized tails

Effect of mutations designed to change TtCutA specificity into PUP. (A) Length distribution for tails added by wild-type (WT) and mutant (PUP1-PUP3) TtCutA_tr3 variants to ss22-A₄ (left) and ss22-U₄ (right) oligoribonucleotide substrates. Numbers of analyzed sequences, obtained after 3′-RACE-seq high-throughput analysis for each protein variant, are indicated in parentheses. (B,C) Analysis of nucleotide composition for tails added by different TtCutA_tr3 variants to ss22-A₄ (panel B) and ss22-U₄ (panel C) RNA substrates. Results are presented as in Figure 6.