Biochemical and structural bioinformatics studies of fungal CutA nucleotidyltransferases explain their unusual specificity toward CTP and increased tendency for cytidine incorporation at the 3′-terminal positions of synthesized tails

Different fractions of tails synthesized by WT, PAP1, and PUP3 variants of TtCutA_tr3 contain a given number of cytidines or uridines. Upper graph shows percentage of tails derived from the 3′-RACE-seq data presented in Figure 10, which entirely lack cytidine (0xC), or contain at least one single cytidine or at least one 2- or 3-nt-long string of consecutive cytidines. Lower graph demonstrates analogous results for uridines.