Affinity resins containing enzymatically resistant mRNA cap analogs—a new tool for the analysis of cap-binding proteins

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FIGURE 3.
FIGURE 3.

A Western blot of eIF4E-His6/HA/ProtA and Cbp20-Myc purified from yeast extract using m7GTP-Sepharose, m7GpCH2pp-Sepharose, m7GpppA-Sepharose, eluted with 1 mM m7GTP and detected with PAP and anti-Myc antibodies, respectively. Western blot was performed on total lysate (Total), flow trough (FT), and elutions from each resin.

This Article

  1. Published in Advance May 15, 2012, doi: 10.1261/rna.032078.111 RNA 18: 1421-1432

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