Affinity resins containing enzymatically resistant mRNA cap analogs—a new tool for the analysis of cap-binding proteins

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FIGURE 2.
FIGURE 2.

Binding of recombinant mouse eIF4E(28–217) and human DcpS to m7GTP-Sepharose (1), m7GpCH2pp-Sepharose (2), m7GpppA-Sepharose (3), and m7GpCH2ppA-Sepharose (4). Coomasie blue-stained SDS-PAGE of total, flow-through (FT), and bound fractions eluted with 1 mM m7GTP.

This Article

  1. Published in Advance May 15, 2012, doi: 10.1261/rna.032078.111 RNA 18: 1421-1432

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