(A) The intronic polyadenylation site is regulated by the NCR. (Left) Diagrams of the relevant regions of rem1/nmt81 chimeras assayed for polyadenylation within the intron. (Right) The same RNA preparations assayed in Figure 5 were used to assay intronic polyadenylation (lanes 1–6). As negative and positive controls, the assay was also carried out on RNA extracted from vegetative (0-h time point) and meiotic (5-h time point) cells, respectively. Sequence analysis indicates that the product marked with an asterisk is derived from rpl2001/2002. It is absent from the meiotic sample because the transcript accumulates only in vegetative cells (Mata et al. 2002). (B) Effects of splice site mutations on rem1 RNA processing. The alleles assayed are diagrammed at the left. The results of RNA processing assays are shown at right, with splicing in the top panel, intronic polyadenylation in the middle, and polyadenylation at the +88 site at the bottom.
