(A) Splicing (top) of both introns in the spo4 gene proceeds in parallel with polyadenylation (bottom) over a meiotic time course. RNA processing reactions were assayed as shown in the diagram beneath each gel image; the minor bands migrating between unspliced spo4 precursor and fully spliced spo4 mRNA may be derived from partially spliced products, but their abundance is insufficient for sequence analysis. (B) The constitutively expressed SPAC6F6.11c gene is fully processed throughout the meiotic time course. In the splicing assay (top), the predominant product observed at all time points lacks all four introns; the minor bands migrating more slowly than fully spliced SPAC6F6.11c RNA may reflect partial splicing, which could not be verified by sequence analysis. The major and minor 3′ RNA processing sites detected in the polyadenylation assay (bottom) were reported in a previous study (Cremona et al. 2011).
