An energetically beneficial leader–linker interaction abolishes ligand-binding cooperativity in glycine riboswitches

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FIGURE 4.
FIGURE 4.

(A) Native gel image of 32P-labeled VCII133 titrated with aptamer I constructs in 10 mM MgCl2 and 5 mM glycine. (Lane 1) 32P-labeled VCLD; (lane 2) 32P-labeled VCII133; (lanes 3–8) 32P-labeled VCII133 with 0.2–97 μM VCI132; (lanes 9–21) 32P-labeled VCII133 with 0.2–100 μM VCI132LD. (B) Semi-log plots of unnormalized fraction of VCII133 bound versus aptamer I concentration.

This Article

  1. Published in Advance January 25, 2012, doi: 10.1261/rna.031286.111 RNA 18: 496-507

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