An energetically beneficial leader–linker interaction abolishes ligand-binding cooperativity in glycine riboswitches

In-line probing of full-length and truncated glycine riboswitches. (A) Representative in-line probing gel image of BSLD. NR, T1, and OH represent no reaction, partial digestion with RNase T1, and partial digestion with alkali, respectively. r2–r7 indicate regions defined in Table 2. (B) Representative semi-log plots of the normalized fraction of RNA bound versus glycine concentration. Shown from top to bottom: VCLD/VCIII (r3); FNLD/FNIII (r6); BSLD/BSIII (r6).