
Distribution of nontemplated THP lengths. The THP lengths were counted for reads with nontemplated 3′-end nucleotides. Overall, 3.2 million reads covering 11,616 genes were collapsed to 82,176 unique sequences. The cumulative frequency of THP lengths of this collapsed data set is shown; that is, if an RNA had a THP of five uridines, it was included in the totals for lengths of one through five. The expected line is the probability of finding a homopolymer of a given length at random, based on equal representation of all nucleotides.










