The nontemplated 3′ ends of small RNAs in human ES cells. (A) The distribution of paired-end-2 sequencing primer reads by RNA gene class. The distribution for all uniquely aligned reads (left) and those reads with nontemplated 3′-end THP (right) are shown. (B–F) The frequency at which each nucleotide was found as the nontemplated THP was calculated for each RNA gene class. The first column is the percent of reads for a specific gene or gene class that had nontemplated 3′ ends. The numbers in the (MM) column are the number of reads with mismatches between the THP and the reference genome. (C) The overall frequency of nontemplated 3′ ends for several classes of RNA genes. Shown are the most highly sequenced genes of miRNAs (B), tRNA anti-codons (D), snRNAs (E), and snoRNAs (F).
